| 出品公司: | Promega |
|---|---|
| 载体名称: | pNL1.3.CMV[secNluc/CMV] |
| 质粒类型: | 荧光素酶表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | -- |
| 启动子: | CMV |
| 载体大小: | 3945 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 在强CMV启动子控制下表达分泌型NanoLuc®荧光素酶的共转染载体。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
![pNL1.3.CMV[secNlucCMV]载体图谱](/uploads/载体图谱/pNL1.3.CMV[secNlucCMV]载体图谱.png)
载体简介
载体序列
LOCUS pNL1.3.CMV[secNluc CMV] 3945 bp ds-DNA circular SYN 26-JUL-2013
DEFINITION Co-transfection vector for expressing secreted NanoLuc(R) luciferase
under control of the strong CMV promoter.
ACCESSION JQ513378
VERSION .
KEYWORDS pNL1.3.CMV[secNluc CMV]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3945)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Saturday, June 6, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3945
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 227..530
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 531..734
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 859..945
/codon_start=1
/product="secretion signal from interleukin 6"
/note="IL-6 signal sequence"
/translation="MNSFSTSAFGPVAFSLGLLLVLPAAFPAP"
CDS 946..1458
/codon_start=1
/product="NanoLuc(R) luciferase"
/note="Nluc"
/note="human codon-optimized"
/translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
LCERILA"
polyA_signal 1499..1620
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2039..2627)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2827..3687)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 3792..3840
/note="synthetic polyadenylation signal"
misc_feature 3854..3945
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gcctcaatat tggccattag ccatattatt cattggttat atagcataaa
61 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
121 ttggctcatg tccaatatga ccgccatgtt ggcattgatt attgactagt tattaatagt
181 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
241 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
301 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
361 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
421 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
481 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
541 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
601 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
661 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
721 atataagcag agctcgttta gtgaaccgtc agatcactag aagctttatt gcggtagttt
781 atcacagtta aattgctaac gcagtcagtg ggcctcggcg gccaagcttg gcaatccggt
841 actgttggta aagccaccat gaactccttc tccacaagcg ccttcggtcc agttgccttc
901 tccctgggcc tgctcctggt gttgcctgct gccttccctg ccccagtctt cacactcgaa
961 gatttcgttg gggactggcg acagacagcc ggctacaacc tggaccaagt ccttgaacag
1021 ggaggtgtgt ccagtttgtt tcagaatctc ggggtgtccg taactccgat ccaaaggatt
1081 gtcctgagcg gtgaaaatgg gctgaagatc gacatccatg tcatcatccc gtatgaaggt
1141 ctgagcggcg accaaatggg ccagatcgaa aaaattttta aggtggtgta ccctgtggat
1201 gatcatcact ttaaggtgat cctgcactat ggcacactgg taatcgacgg ggttacgccg
1261 aacatgatcg actatttcgg acggccgtat gaaggcatcg ccgtgttcga cggcaaaaag
1321 atcactgtaa cagggaccct gtggaacggc aacaaaatta tcgacgagcg cctgatcaac
1381 cccgacggct ccctgctgtt ccgagtaacc atcaacggag tgaccggctg gcggctgtgc
1441 gaacgcattc tggcgtaatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata
1501 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
1561 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
1621 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
1681 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc
1741 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
1801 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt
1861 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
1921 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
1981 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
2041 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
2101 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
2161 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
2221 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
2281 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
2341 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
2401 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
2461 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
2521 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
2581 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
2641 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
2701 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
2761 caatctaaag tatatatgag taaacttggt ctgacagcgg ccgcaaatgc taaaccactg
2821 cagtggttac cagtgcttga tcagtgaggc accgatctca gcgatctgcc tatttcgttc
2881 gtccatagtg gcctgactcc ccgtcgtgta gatcactacg attcgtgagg gcttaccatc
2941 aggccccagc gcagcaatga tgccgcgaga gccgcgttca ccggcccccg atttgtcagc
3001 aatgaaccag ccagcaggga gggccgagcg aagaagtggt cctgctactt tgtccgcctc
3061 catccagtct atgagctgct gtcgtgatgc tagagtaaga agttcgccag tgagtagttt
3121 ccgaagagtt gtggccattg ctactggcat cgtggtatca cgctcgtcgt tcggtatggc
3181 ttcgttcaac tctggttccc agcggtcaag ccgggtcaca tgatcaccca tattatgaag
3241 aaatgcagtc agctccttag ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt
3301 gtcgctcatg gtaatggcag cactacacaa ttctcttacc gtcatgccat ccgtaagatg
3361 cttttccgtg accggcgagt actcaaccaa gtcgttttgt gagtagtgta tacggcgacc
3421 aagctgctct tgcccggcgt ctatacggga caacaccgcg ccacatagca gtactttgaa
3481 agtgctcatc atcgggaatc gttcttcggg gcggaaagac tcaaggatct tgccgctatt
3541 gagatccagt tcgatatagc ccactcttgc acccagttga tcttcagcat cttttacttt
3601 caccagcgtt tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga agggaatgag
3661 tgcgacacga aaatgttgga tgctcatact cgtccttttt caatattatt gaagcattta
3721 tcagggttac tagtacgtct ctcaaggata agtaagtaat attaaggtac gggaggtatt
3781 ggacaggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
3841 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa
3901 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctct
//
